منابع مشابه
Enzymatic Peptide Synthesis. Carboxypeptidase Y Catalyzed Formation of Peptide Bonds
It is demonstrated that carboxypeptidase Y from Saccharomyces cerevisiae can catalyze the formation of peptide bonds using N-acylamino acid esters as substrates and free amino acids or amino acid amides as nucleophiles, The coupling yields observed with free amino acids were max. 60 % for alanine and lysine and they depended strongly on the reaction parameters; viz. pH, temperature and concentr...
متن کاملPeptide synthesis: chemical or enzymatic
Abbreviations: CD: circular dichroism CLEC: cross linked enzyme crystals DDC: double dimer constructs ESI: electrospray ionization HOBT: hydroxybenzotriazole HPLC: high performance liquid hromatography KCS: kinetically controlled synthesis MALDI: matrix-assisted laser desorption ionization MAP: multiple antigen peptide system MS: mass spectrometry NMR: nuclear magnetic resonance SPS: solution p...
متن کاملEnzymatic Synthesis of the Peptide in Bacterial Uridine Nucleotides
The pentapeptide moiety of uridine diphosphate acetylmuramyl-pentapeptide, a precursor of the peptidoglycan of bacterial cell walls, is synthesized in a reaction sequence involving five enzymes. The amount and specificity of these enzymes have been studied in 10 microorganisms, five of which contain L-lysine as the third amino acid in the peptide sequence, and five of which contain instead meso...
متن کاملcomparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.
heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولSimple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Trypsin from the pyloric caeca of Pacific cod (Gadus macrocephalus) was easily prepared by affinity chromatography on Benzamidine Sepharose 6B and gel filtration on Superdex 75. Pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 Da, 23,939.62 Da, and 24,114.81 Da by desorption/ionization time-of-flight mass spectroscopy (MALDI/TOF-MS) and the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1938
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)74064-7